【文题】Selective reduction of chromogranin A-like immunoreactivities in the prefrontal cortex of schizophrenic subjects: a postmortem study
【杂志】Neuroscience Letters 367 (2004) 293–297
【作者】Takeshi Iwazaki a, Isao Shibata a, Shin-Ichi Niwa a, Izuru Matsumoto b,.
【单位】a Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Hikarigaoka 1 960-1295, Japan b Department of Pathology, University of Sydney, Blackburn (D06), Sydney, NSW 2006, Australia
【文摘】It is suggested that secretogranin s/chromogranins play a role in regulating secretion of various proteins and amines, including neurotransmitters from secretory granules. Several studies have implicated the importance of altered synaptic connectivity in schizophrenia. We employed immunohistochemical techniques to determine if the level of chromogranin A (CgA)-immunoreactivity (IR) was altered in the subjects with schizophrenia. Nine subjects with schizophrenia and nine age- and sex-matched control subjects were selected for this study.
Immunohistochemistry using specific antibody against CgA was performed on sections of prefrontal cortex and hippocampus. Images of CgA-IR were analyzed by computer-based image analyzing software. CgA-IR was significantly decreased in layers III–V of the prefrontal cortex in schizophrenic subjects compared with control subjects. In the hippocampus, no significant difference was observed between two groups. The results indicate that there may be a decrease in the number of CgA positive large dense-core vesicles per terminal, and/or in the number of CgA positive terminals, suggesting possible functional impairment of prefrontal synaptic contact in schizophrenia.
【翻译】有研究提示分泌粒蛋白/嗜铬粒蛋白在调节不同的蛋白质和胺类(包括从分泌颗粒中释放的神经递质)的分泌上起作用。一些研究已经认识到精神分裂症中突触连接改变的重要性。我们采用免疫组化技术研究精神分裂症病人嗜铬粒蛋白 A(CgA)-免疫活性(IR)是否发生改变。九个精神分裂症病人和九个年龄和性别相应的正常对照者参与本次实验。在前皮层和海马脑片中加入特异性抗CgA抗体，进行免疫组化实验。用计算机成象分析软件对CgA-IR成象进行分析。与对照组相比，精神分裂症组前皮层III–V层CgA-IR明显降低。而在海马两组CgA-IR无明显差异。结果提示精神分裂症病人CgA阳性的神经末梢数或每个末梢的CgA阳性大致密核囊泡数减少，说明精神分裂症病人前皮层突触连接可能存在功能上的障碍。
转自 ziyu00
