【文题】Immunohistochemical and immunoblot study of GABAA α1 and β2/3 subunits in the prefrontal cortex of subjects with schizophrenia and bipolar disorder
【杂志】Neuroscience Research 50 (2004) 77-84
【作者】Masanori Ishikawa, Katsuyoshi Mizukami., Masahiko Iwakiri, Shin Hidaka, Takashi Asada
【单位】Department of Psychiatry, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba City, Ibaraki 305-8575, Japan

【文摘】A number of investigations have provided a growing body of evidence of the involvement of the γ-aminobutyric acid (GABA) transmitter system in the pathophysiology of schizophrenia and bipolar disorder. In this study, immunohistochemical and immunoblot techniques were employed in order to examine alterations of the GABAA receptor α1 and β2/3 subunits in the prefrontal cortex from postmortem subjects with schizophrenia and bipolar disorder. α1 immunoreactivity was observed in the neuropil of the prefrontal cortex and in the neuronal soma in specimens from both groups, as well as from normal controls. α1 immunolabeling in the neuronal soma from the schizophrenic group was more intense than that of the other two groups. The distribution of β2/3 immunoreactivity was similar to that of α1. β2/3 immunolabeling in the neuronal soma from the schizophrenia and bipolar disorder groups was more intense than that of the normal controls. The densitometry measurements, as well as the immunoblot analysis for α1 and β2/3 were highly consistent with the α1 and β2/3 immunohistochemistry results. The present study suggests that the expression of these two GABAA receptor subunits was altered in subjects with schizophrenia and bipolar disorder, but that the patterns of change differed between those with these two disorders. 

【翻译】越来越多的研究提供证据表明γ-氨基丁酸（GABA）递质系统参与精神分裂症和双相障碍的病理生理过程。本实验采用免疫组化和免疫印记技术研究精神分裂症和双相障碍患者死后尸检脑前皮层GABAA受体α1和β2/3 亚单位的改变。在观察两组病人前皮层神经毡和神经元胞体中α1亚单位免疫反应性改变的同时对正常对照组也进行相同的考察。结果表明精神分裂症病人神经元胞体中α1免疫标记明显强于其它两组。β2/3免疫活性部位分布与α1相似。 精神分裂症和双相障碍两组病人神经元胞体中β2/3免疫标记部位的反应强度均明显高于正常对照组。光密度测定法和免疫印记法对α1 和 β2/3亚单位的分析结果与免疫组化结果高度一致。本研究表明精神分裂症和双相障碍病人GABAA受体α1和β2/3 两种亚单位发生改变，但改变形式有所不同。

转自  ziyu00